FastQCFastQC Report
Thu 2 Jun 2016
DRR028189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918366
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC14850.059.062267145
CGACGAA8450.052.326817145
GGTATCA19450.048.0752221
TCAACGC26850.046.433924
ATCAACG27800.044.847153
GTATCAA30400.044.8265651
GTACATG62250.041.104231
CAACGCA31750.039.2677425
TACATGG65300.036.6312682
AACGCAG34500.036.5579076
TATCAAC37200.034.878782
ACATGGG68200.033.373093
ACGCAGA38400.032.467477
CGCAGAG39200.031.0652188
TAGACCG1204.06418E-430.2022955
TGACGCG2252.8254362E-828.994204145
CATGGGG48150.027.8500924
GAGTACA45950.024.9242881
GCAGAGT47450.023.9836129
AGTACAT48350.023.0874212
Produced by FastQC (version 0.11.5)