FastQCFastQC Report
Thu 2 Jun 2016
DRR028189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918366
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.14361180296147866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.13464583921941903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.076.3548051
GTATCAA51500.056.524681
ATCAACG59850.047.2415123
TCAACGC60050.047.084174
CAACGCA62850.044.986555
AACGCAG66250.042.8966646
TATCAAC67300.042.1196752
ACGCAGA74650.037.6812447
GTACATG60700.037.0743871
TACATGG60600.035.8898772
CGCAGAG77200.035.8731428
ACATGGG62950.033.7438963
GTGGTAT16200.030.9196871
GCAGAGT88400.030.6720439
TGGTATC17700.028.2617572
CATGGGG49050.027.3436224
GAGTACA43950.025.2716481
CTCCGAC6950.023.996372145
GTATAGG7750.023.4174371
TCGTATC2802.341585E-723.307035145
Produced by FastQC (version 0.11.5)