FastQCFastQC Report
Thu 2 Jun 2016
DRR028188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241193
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18150.050.3255044
ATCAACG18800.048.5855263
GTATCAA20400.046.9070321
CAACGCA20200.045.2182125
AACGCAG21600.042.9586336
GGTATCA12850.041.746761
TATCAAC24400.038.9202542
ACGCAGA24900.036.6830447
CGCAGAG25200.035.6710058
GTACATG38650.035.074081
TACATGG39650.032.909672
GTCTCGC2755.456968E-1231.6331731
ACATGGG42800.030.487583
GCAGAGT30200.028.3249579
GAGTACA28800.026.6813351
CATGGGG31700.026.0699274
GGATAGG10950.025.819313145
AGTACAT30950.022.9540672
GTACGGA1700.003095020521.3213796
CCGCGTG2404.3673666E-521.14379
Produced by FastQC (version 0.11.5)