FastQCFastQC Report
Thu 2 Jun 2016
DRR028188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241193
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.13527308001253632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.12729688291828908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.065.139591
GTATCAA31450.051.7085341
TCAACGC35500.046.1532364
ATCAACG35700.045.6916053
CAACGCA37450.043.7500655
AACGCAG39500.041.8465656
TATCAAC41700.039.81592
CTCCGAC5050.037.33439145
ACGCAGA44450.037.023397
GTGGTAT9150.035.7047731
CGCAGAG46500.034.7675448
GTACATG38450.030.5881731
GCAGAGT52200.030.5544349
TACATGG39600.029.2942162
ACATGGG39800.027.6874263
GAGTACA27300.026.0614321
TGGTATC11800.024.577352
CATGGGG29950.023.7220024
CAGAGTA50950.023.6203339
AGTACAT30100.022.8830662
Produced by FastQC (version 0.11.5)