FastQCFastQC Report
Thu 2 Jun 2016
DRR028187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418573
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21550.046.0827334
ATCAACG21700.045.09613
GGTATCA15150.044.975931
GTATCAA23450.044.8218841
CAACGCA24250.040.9518745
AACGCAG25600.039.6417666
TATCAAC27000.037.049322
ACGCAGA28500.035.0993587
GTACATG43300.033.8164831
CGCAGAG29950.033.4000558
TACATGG43700.031.6823142
ACATGGG47050.028.8102443
TGACGCG1658.4306484E-526.359201145
CATGGGG34750.025.6575534
GCAGAGT37400.025.3901149
GAGCGAC8250.024.601923145
TCTCGGG13800.024.162603145
GTACGGA1500.001496045624.1626011
GAGTACA33350.023.0396021
GCGATCA5101.8189894E-1221.319942145
Produced by FastQC (version 0.11.5)