FastQCFastQC Report
Thu 2 Jun 2016
DRR028187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418573
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.11116805409379707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.11018114682853826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.072.082811
GTATCAA34200.053.71461
TCAACGC36700.048.7926864
ATCAACG37200.048.136873
CAACGCA39000.045.9151735
AACGCAG41400.043.428546
TATCAAC43850.041.0020872
ACGCAGA48050.036.8146177
CGCAGAG49000.036.100878
GTACATG36750.033.3908351
GCAGAGT57150.030.3183489
TACATGG38850.029.8574582
GTCGTAC1000.006030366829.0440981
GTGGTAT13000.026.8099381
ACATGGG42100.026.6915283
TTGTGCG1658.422877E-526.3627769
GAGTACA29850.026.2710441
CTCCGAC6250.025.526367145
GTCTCGC3851.6370905E-1124.5177461
TGGTATC13600.023.4551162
Produced by FastQC (version 0.11.5)