FastQCFastQC Report
Thu 2 Jun 2016
DRR028186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523148
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.058.492371
GACGACC13500.052.0858145
GTATCAA22800.044.1937981
TCAACGC21800.043.893364
ATCAACG22900.041.7849433
CAACGCA25450.037.598245
GTACATG46950.036.901471
AACGCAG26150.036.8689966
TACATGG49250.034.000642
ACGCAGA29600.031.8370657
ACATGGG52100.031.4450343
TATCAAC30950.031.3852442
CCGACGC5700.030.522335145
CGCAGAG31900.029.5416038
CATGGGG37050.028.5657754
CTTACGG6050.026.360199145
GGACGGT2101.5573341E-524.1635176
GCAGAGT40000.023.0157479
TCTCGGG15950.022.72431145
GAGTACA34950.021.7782751
Produced by FastQC (version 0.11.5)