FastQCFastQC Report
Thu 2 Jun 2016
DRR028186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523148
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.11259575563241392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.10773739649725436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.075.4653241
GTATCAA36650.051.306511
ATCAACG39300.047.5933273
TCAACGC39300.047.5933274
CAACGCA41600.044.9619675
AACGCAG44000.042.674266
TATCAAC45850.041.111872
ACGCAGA51200.036.248417
CGCAGAG52250.035.2424748
GTACATG43550.032.8416331
TACATGG43250.031.51412
CTCCGAC6550.031.001183145
GCAGAGT63250.029.1133449
ACATGGG46200.027.3039973
GTGGTAT12850.025.9897081
CCGTTAC1409.999559E-425.891727
TGGTATC14100.023.138042
CATGGGG36100.023.0945514
GTACGAC1952.6163924E-422.3067113
GAGTACA34350.021.3472211
Produced by FastQC (version 0.11.5)