FastQCFastQC Report
Thu 2 Jun 2016
DRR028184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324387
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG6750.052.61928145
TCAACGC31050.046.923144
ATCAACG32300.045.5560573
GTATCAA33400.045.357851
GGTATCA22150.043.8514251
CAACGCA35550.040.9835055
TCTCGGG20900.039.884502145
GTACATG74900.038.420351
AACGCAG39700.037.0645036
TACATGG74950.036.6539042
TATCAAC42100.035.1237372
ACGCAGA43950.033.1504827
ACATGGG83450.032.0518153
CGCAGAG46050.031.0091048
CATGGGG62450.026.463974
CTTAGGG32000.025.596529145
GCAGAGT55600.024.770319
GAGTACA55800.024.0320131
AGTACAT58300.022.1311552
AGAGTAC49950.018.7200455
Produced by FastQC (version 0.11.5)