FastQCFastQC Report
Thu 2 Jun 2016
DRR028184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324387
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.071.190361
GTATCAA56450.058.264431
TCAACGC65100.050.1136134
ATCAACG65400.049.883733
CAACGCA69450.046.8703585
TATCAAC73600.044.2275282
AACGCAG73900.044.0479856
ACGCAGA83200.038.9500777
CGCAGAG83850.038.388768
GTACATG74250.036.864991
TACATGG75550.034.0657582
GCAGAGT97650.032.666659
ACATGGG78550.031.472583
CTCCGAC10100.030.15469145
GAGTACA56900.026.7963891
GTGGTAT17700.025.842621
CATGGGG58800.025.0289654
TCGTATC3551.546141E-1024.512062145
AGTACAT58700.024.4540772
CTAGCGT1500.001495144724.1658974
Produced by FastQC (version 0.11.5)