FastQCFastQC Report
Thu 2 Jun 2016
DRR028183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485413
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.048.8158761
GTATCAA22100.047.229511
TCAACGC20550.046.206464
ATCAACG21000.045.5614853
CGACGAC8150.042.690067145
CAACGCA22550.041.786895
AACGCAG24800.038.2880136
GTACATG46750.036.1258051
TATCAAC29650.033.4918442
TACATGG48000.033.0709082
ACGCAGA28950.032.0482757
ACATGGG50900.030.4746843
ATCTACC14350.029.801857145
CGCAGAG30650.029.5612478
TCTCGGG15800.028.44316145
TGACGCG2751.9741128E-723.722095145
GCAGAGT37700.023.456429
CATGGGG39750.023.3408164
GAGTACA33600.023.082761
GGACCGT2953.8851613E-722.113826
Produced by FastQC (version 0.11.5)