FastQCFastQC Report
Thu 2 Jun 2016
DRR028183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485413
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.070.905131
GTATCAA36000.051.823581
TCAACGC38200.046.6872254
ATCAACG38500.046.135123
CAACGCA40250.043.9491275
AACGCAG43150.041.3314486
TATCAAC44950.040.3228532
ACGCAGA48900.036.323157
GTACATG47500.035.91461
CGCAGAG50250.035.347318
TACATGG50750.031.1430662
GTGGTAT13050.030.5948981
ACATGGG52200.028.610343
GCAGAGT60700.028.4259229
CTCCGAC5850.027.270647145
TGGTATC15500.024.7904872
GAGTACA36700.024.5274521
CATGGGG38900.023.2962864
AGTACAT38900.022.7379422
ATGCCGC3809.029463E-920.991222145
Produced by FastQC (version 0.11.5)