FastQCFastQC Report
Thu 2 Jun 2016
DRR028182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573216
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.047.7818831
TCAACGC23450.041.731764
ATCAACG23750.041.204623
GTATCAA24450.040.3214151
GTACATG47400.038.5387761
CAACGCA26500.037.2022135
AACGCAG26700.036.9235466
TACATGG48550.034.3411142
ACATGGG50200.032.345963
ACGCAGA30200.032.1642657
TATCAAC30700.032.112662
CGACGAA4650.031.178326145
ACGATAG2807.2759576E-1231.066975145
CGCAGAG31850.029.3599978
CATGGGG39450.026.4600834
GTCGTAC2351.3329118E-624.6773151
GCAGAGT38100.022.8313739
TGACGCG1952.6182062E-422.304495145
GAGTACA35050.021.7158581
ACCGACC3457.5226126E-821.011488
Produced by FastQC (version 0.11.5)