FastQCFastQC Report
Thu 2 Jun 2016
DRR028182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573216
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.13354809511217786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.12217012794174481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.065.466521
GTATCAA39250.054.1920051
ATCAACG44200.047.0731163
TCAACGC44550.046.7032974
CAACGCA47350.043.941545
AACGCAG48750.042.530926
TATCAAC50200.041.3024372
ACGCAGA56600.036.3760267
CGCAGAG57600.035.4927758
GTACATG49350.030.1560151
GCAGAGT67150.030.0131879
TACATGG49350.029.0864932
GTGGTAT13800.028.4067751
ACATGGG50650.027.9105573
CTCCGAC5850.026.03575145
GTATACG1701.0225108E-425.62181
GAGTACA38750.023.97981
TGGTATC15800.023.400572
CAGAGTA65900.022.1117959
CATGGGG40500.022.0172674
Produced by FastQC (version 0.11.5)