FastQCFastQC Report
Thu 2 Jun 2016
DRR028181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933449
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC15200.065.333595145
TGACGCG1800.052.35166145
GGTATCA19900.051.724331
TCAACGC26000.043.7709474
ATCAACG26300.043.547273
CGACGAA7600.041.966106145
GTATCAA30600.039.3229641
CAACGCA30350.037.7361875
GTACATG56600.037.5241471
AACGCAG32800.034.9174776
TACATGG60350.033.6310462
TATCAAC36800.032.1069762
ACGCAGA34800.031.869257
ACATGGG65100.030.5090853
CGCAGAG36300.030.152968
CATGGGG49050.025.8617944
GACGATC4750.024.416643145
TCTCGGG22750.024.215408145
GTCGTAC2101.5582207E-524.1623061
GAGTACA45200.023.7346531
Produced by FastQC (version 0.11.5)