FastQCFastQC Report
Thu 2 Jun 2016
DRR028181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933449
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.066.000961
GTATCAA44800.058.0160181
ATCAACG50450.051.589113
TCAACGC50900.051.133024
CAACGCA52150.049.907395
AACGCAG54950.047.364346
TATCAAC57950.044.7895622
ACGCAGA64550.039.646357
CGCAGAG65500.038.9606488
GTACATG56550.033.251411
GCAGAGT77550.032.065439
TACATGG57350.031.0991782
ACATGGG59750.029.2417873
CTCCGAC7400.027.437273145
GAGTACA42100.025.3500121
CATGGGG44400.023.3495124
AGTACAT45450.022.3327062
ATATACG2404.366618E-521.1451873
TCGTATC3457.4995114E-821.0182145
CAGAGTA74800.020.7413449
Produced by FastQC (version 0.11.5)