FastQCFastQC Report
Thu 2 Jun 2016
DRR028180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014269
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.053.7527281
TCAACGC29150.048.9901284
GTATCAA31250.047.7857171
ATCAACG30150.047.6056823
CAACGCA34350.041.78495
AACGCAG36300.039.5402576
GTACATG66100.035.7517281
TATCAAC41550.034.7186622
ACGCAGA40950.034.3422477
TACATGG67400.032.5884442
CGCAGAG42700.032.0859458
ACATGGG72850.028.5583573
GAGTACA48450.027.3803021
TGACGCG2259.12567E-725.774391145
GCAGAGT51050.025.2758149
CATGGGG54300.025.231494
GGATAGG16600.024.891308145
AGTACAT51750.023.9533732
GTATTAG15250.021.3906291
TCTCGGG24150.021.311897145
Produced by FastQC (version 0.11.5)