FastQCFastQC Report
Thu 2 Jun 2016
DRR028180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014269
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.12128469434817296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.11403640725245734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.070.979581
GTATCAA51550.058.304061
ATCAACG59550.049.427343
TCAACGC59850.049.1795854
CAACGCA61650.047.7436875
AACGCAG64900.045.2411166
TATCAAC69800.042.5855452
ACGCAGA75550.038.5757687
CGCAGAG76450.038.0268068
GTACATG63050.032.355521
GCAGAGT89400.031.9507969
TACATGG63750.030.2506832
GTGGTAT17550.028.5429651
ACATGGG67950.027.846723
TGGTATC18050.026.1077772
TCGTATC4001.8189894E-1225.379793145
GAGTACA49200.023.9043561
CAGAGTA86450.022.6423659
CATGGGG53800.021.9648554
AGTACAT51050.021.3024372
Produced by FastQC (version 0.11.5)