FastQCFastQC Report
Thu 2 Jun 2016
DRR028179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640337
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41150.047.385481
TCAACGC38350.046.6868364
ATCAACG38400.046.6260453
GGTATCA27400.043.386651
TGACGCG2800.041.421402145
CAACGCA44850.040.2438745
AACGCAG49400.036.5371976
TATCAAC51250.036.3498082
GTACATG88450.034.9939421
TACATGG90200.032.7077522
ACGCAGA54650.032.3640257
GAGCGAC18350.031.20713145
CGCAGAG56950.030.8023958
ACATGGG97500.028.9206353
TCTCGGG29300.027.955912145
GAGTACA68700.025.8506951
CATGGGG70150.024.6963674
GCAGAGT70350.023.8018519
AGTACAT71700.023.25262
TAGTACG5200.022.3038334
Produced by FastQC (version 0.11.5)