FastQCFastQC Report
Thu 2 Jun 2016
DRR028179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640337
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.068.581181
GTATCAA60650.058.657421
ATCAACG70750.049.083223
TCAACGC71250.048.738784
CAACGCA74400.046.675245
AACGCAG78300.044.350426
TATCAAC82300.043.1654932
ACGCAGA92550.037.521757
CGCAGAG93850.036.8475078
GTACATG86050.034.171331
GCAGAGT111300.030.3539169
TACATGG90450.030.2185232
ACATGGG92050.028.9045543
CTCCGAC10400.027.192802145
GAGTACA67950.025.2162381
CATGGGG69100.023.9210824
AGTACAT70650.023.4997792
CAGAGTA109250.022.5622029
GTGGTAT22700.021.7490751
AGAGTAC100100.019.1202625
Produced by FastQC (version 0.11.5)