FastQCFastQC Report
Thu 2 Jun 2016
DRR028178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544395
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC15000.056.537056145
GGTATCA16450.048.4691471
TCAACGC22800.045.1431734
ATCAACG23300.043.863353
GTATCAA25500.043.2057951
CAACGCA26450.038.9135865
AACGCAG28300.037.138146
GTACATG48450.036.3539051
TATCAAC31250.034.328142
TACATGG49750.033.6556132
ACGCAGA31350.032.3689887
ACATGGG53150.030.6844163
CGCAGAG33300.029.8205018
GCAGAGT40450.024.728589
CATGGGG40150.024.3717564
GAGTACA36400.022.3025881
TGACGCG1952.6196908E-422.302588145
CCGACGC6500.021.187458145
AGAGTAC32000.021.065495
CATGGGA19350.020.2279284
Produced by FastQC (version 0.11.5)