FastQCFastQC Report
Thu 2 Jun 2016
DRR028178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544395
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.16582545268535576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23970.15520640768715258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.081.062821
GTATCAA44100.053.9893651
ATCAACG50850.047.3337973
TCAACGC51450.046.78184
CAACGCA54600.043.950075
AACGCAG56900.042.173536
TATCAAC57350.041.71622
ACGCAGA65850.036.2213257
CGCAGAG66050.036.0018848
GTACATG49900.035.49461
TACATGG52600.032.8031432
GCAGAGT74400.031.4741349
ACATGGG53900.031.0704423
CTCCGAC6700.030.304413145
GTGGTAT14800.028.937651
GATACCG1701.0318711E-425.5874259
TGGTATC14850.024.412
CATGGGG40200.024.3462434
CAGAGTA73350.023.0292639
GAGTACA36850.021.865451
Produced by FastQC (version 0.11.5)