FastQCFastQC Report
Thu 2 Jun 2016
DRR028176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559354
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.058.6790051
GTATCAA29100.046.3311461
ATCAACG28650.045.287833
TCAACGC28850.044.9738774
TGACGCG5450.043.890503145
CAACGCA31550.041.5845765
AACGCAG33350.039.1227846
GTACATG56950.037.929371
TATCAAC35100.037.5852432
TACATGG56000.036.5017932
GTCGTAC1301.657589E-533.4549941
ACGCAGA38700.032.9651347
TCTCGGG14350.031.823048145
CGCAGAG39400.031.643568
ACATGGG63850.029.6300723
TCTGTCG6550.026.5596928
GCAGAGT43500.026.4948189
CATGGGG47500.025.6370944
CTTAGGG19250.025.228832145
GTGGTAT13000.024.5336651
Produced by FastQC (version 0.11.5)