FastQCFastQC Report
Thu 2 Jun 2016
DRR028176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559354
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.19027109944246143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.18655161047459395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.10510762790232366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.078.62971
GTATCAA50000.052.258511
ATCAACG58900.043.94163
TCAACGC59200.043.596464
CAACGCA62100.041.560565
TATCAAC65000.039.9293822
AACGCAG65800.039.1133846
GTGGTAT14950.037.868491
ACGCAGA74900.034.4580847
GTACATG55050.034.4118271
TGGTATC15850.033.8474042
CGCAGAG76000.033.7685628
TACATGG54500.032.856662
GCAGAGT83300.030.0259699
ACATGGG60050.029.4577643
CATGGGG44250.027.6883054
CTCCGAC6950.027.128317145
CAGAGTA82300.023.7841079
GAGTACA43750.023.5578061
AGTACAT44250.021.954042
Produced by FastQC (version 0.11.5)