FastQCFastQC Report
Thu 2 Jun 2016
DRR028174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133458
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.051.636241
GTATCAA30950.043.095441
TCAACGC29950.042.8401074
ATCAACG30000.042.7687073
CAACGCA34300.037.6183745
GTACATG66950.037.2462041
AACGCAG37100.035.3654176
TACATGG70800.033.6850172
TATCAAC40450.032.9741362
ACGCAGA41600.031.3655787
ACATGGG74600.030.997453
CGACGAA6150.030.645899145
CGCAGAG44050.029.7856318
TGACGCG2259.127343E-725.773987145
CATGGGG57500.025.2136824
GAGTACA50850.024.9471681
GCAGAGT57900.021.784369
AGTACAT53900.021.2491932
GTATAAC10600.020.515851
GTCGTAC2256.8779784E-419.330491
Produced by FastQC (version 0.11.5)