FastQCFastQC Report
Thu 2 Jun 2016
DRR028174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133458
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.12055545504059606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.11750875808194958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.075.262191
GTATCAA56650.053.706051
TCAACGC63250.047.5679244
ATCAACG63950.046.9338763
CAACGCA66150.045.372965
AACGCAG70100.042.91976
TATCAAC74200.040.2550132
ACGCAGA82300.036.4692767
CGCAGAG84400.035.5618638
GTACATG64100.034.097111
TACATGG68650.030.7311692
GCAGAGT96250.030.5056959
GTGGTAT17850.029.6957131
ACATGGG70550.028.2593613
TGGTATC19050.025.4980222
TATAACG2901.19252945E-824.9993442
ACCGTCC2953.8796134E-722.1180658
CATGGGG54150.022.0908344
CAGAGTA92500.021.6318669
CTCCGAC8150.021.352638145
Produced by FastQC (version 0.11.5)