FastQCFastQC Report
Thu 2 Jun 2016
DRR028173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174813
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC17500.063.375572145
CGACGAA8300.056.76802145
GGTATCA22450.047.7874531
TCAACGC30400.045.5436974
ATCAACG31400.044.5549743
GTATCAA33550.044.0764051
CAACGCA36750.038.2659875
AACGCAG37950.037.0559966
TATCAAC40900.034.7377172
ACGCAGA41600.032.7591827
GTACATG65200.032.575311
CGCAGAG41900.032.005628
TACATGG66700.031.299342
ACATGGG70800.028.1558073
TGACGCG2654.5729394E-927.354113145
CATGGGG53750.023.4660134
GCAGAGT54150.023.4265389
GAGTACA49850.023.2660871
AGTACAT54300.019.4904352
AGAGTAC46350.018.610835
Produced by FastQC (version 0.11.5)