FastQCFastQC Report
Thu 2 Jun 2016
DRR028173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2174813
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.11734342217009004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.11154982060526583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.072.705021
GTATCAA57350.053.4220771
ATCAACG61800.048.3315773
TCAACGC62200.048.137324
CAACGCA66100.045.187475
AACGCAG68050.043.999146
TATCAAC70900.042.3337142
ACGCAGA78400.038.098117
CGCAGAG80700.036.922458
GTACATG65650.031.959861
TCGTATC3000.031.422749145
GCAGAGT94250.030.9219539
TACATGG67750.030.0697082
ACATGGG69700.027.87563
GAGTACA49900.026.6251641
GTGGTAT19200.026.0909331
CATGGGG55200.022.1957534
AGTACAT56700.021.9926512
TGGTATC21800.021.6166862
CAGAGTA88700.020.841979
Produced by FastQC (version 0.11.5)