FastQCFastQC Report
Thu 2 Jun 2016
DRR028171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457366
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22260.48669992959686553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.067.3763351
TGACGCG1051.1114025E-955.214275145
GTATCAA13300.045.7697262
TCAACGC14350.044.9457665
CAACGCA14950.043.626666
ATCAACG14800.043.579174
AACGCAG15750.040.950597
TATCAAC15400.040.469553
GTACATG20300.039.982751
TACATGG20800.038.6732182
CATGGGG13700.034.3829774
ACGCAGA19000.033.945888
CGCAGAG19000.033.5644659
ACATGGG24000.032.9128843
ATGGGGG7750.032.7278145
ATGGGCG1801.2946839E-732.2083245
GTGGTAT4450.029.3131941
TGGTATC4700.029.295872
GCGATCA1753.7814043E-628.987494145
CAAGGGT1555.5101627E-528.0524124
Produced by FastQC (version 0.11.5)