FastQCFastQC Report
Thu 2 Jun 2016
DRR028171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457366
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.8133529820756243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.7890835785782065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.5802792511905126No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.2182059882020089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.20049588294713644No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.12812495900438597No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT5480.11981651456382852No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.10516741515547723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.088.3165361
TGTACCG651.3966019E-766.9213645
GTATCAA28900.060.747631
TCGTGCG400.00604940954.3736049
CTCCGAC2950.051.620136145
GTGGTAT6100.049.9495161
TTCGGCG450.00963679448.3320968
TATCAAC37400.046.5228732
TGGTATC6700.045.4465942
ATCAACG37700.045.3834573
TCGTATC803.727203E-545.321247145
TCAACGC37750.045.131294
CAACGCA38950.043.7408565
AACGCAG40150.042.61416
GCGGTAT700.001052767941.45471
AGACCGA1901.2914825E-1038.16526145
ACGCAGA46250.036.9936457
CGCAGAG47150.036.133758
GCAGAGT49900.033.5612649
TATCTCG1102.4344599E-432.95375