FastQCFastQC Report
Thu 2 Jun 2016
DRR028170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946298
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25900.045.6188774
GTATCAA28150.045.3201181
GGTATCA18600.045.2065961
GACGACC18500.045.059135145
ATCAACG27300.043.279453
CAACGCA29600.039.9165155
GTACATG59650.038.035631
AACGCAG33150.035.64196
TACATGG60200.034.7983062
TATCAAC36600.033.0744132
ACGCAGA36650.031.4470487
ACATGGG66300.030.5033423
CGCAGAG36050.030.3618648
TGACGCG3052.3646862E-1128.519253145
CATGGGG50600.027.2182254
GAGTACA43900.026.9140991
CCGACGC7900.024.773844145
AGTACAT45800.023.8983652
GTATAGG9700.023.1657681
GCAGAGT45600.022.572539
Produced by FastQC (version 0.11.5)