FastQCFastQC Report
Thu 2 Jun 2016
DRR028170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946298
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.10419781554520428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.10080676237657338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.073.017791
GTATCAA46650.061.6169051
TCAACGC53350.053.270074
ATCAACG53500.053.1207163
CAACGCA55250.051.438165
AACGCAG59100.048.209956
TATCAAC62650.045.362462
ACGCAGA68650.040.975367
CGCAGAG70300.039.807388
GTACATG58000.037.795081
GCAGAGT81900.033.638099
TTTCGCG1957.00129E-933.4610449
TACATGG62200.033.452082
CTCCGAC7950.032.830547145
ACATGGG63850.030.430253
GAGTACA45000.024.3568291
CATGGGG48800.023.9187054
GTGGTAT16400.023.4578591
TAGATCG2202.2304604E-523.0678425
CAGAGTA79550.021.6904419
Produced by FastQC (version 0.11.5)