FastQCFastQC Report
Thu 2 Jun 2016
DRR028169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668789
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20850.043.1092383
TCAACGC20950.042.9034654
GACGAAA6350.041.09448145
GTATCAA24100.040.303471
GGTATCA16550.038.1044731
CAACGCA23900.037.607855
GTACATG48600.036.5413481
AACGCAG25050.036.1707086
TACATGG49750.034.3853652
TATCAAC27300.032.9240882
ACGCAGA28550.031.2286877
TGACGCG1652.3912653E-630.751678145
ACATGGG52950.030.6645623
CGCAGAG29750.029.2380878
TCTAGCG1050.00771712327.613752
GCCGACA5550.026.121117145
GTACGAC1450.001228159524.9952073
CATGGGG38800.024.8470384
GAGTACA36700.023.8986871
TAGATCG1700.00309709421.319445
Produced by FastQC (version 0.11.5)