FastQCFastQC Report
Thu 2 Jun 2016
DRR028169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668789
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.11553288043006035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.10840196094293528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.061.4640661
GTATCAA39250.053.1049961
ATCAACG41400.049.557663
TCAACGC41900.048.793264
CAACGCA44550.046.2163165
AACGCAG47200.044.0823366
TATCAAC49400.041.532132
ACGCAGA54100.038.3267
GTACATG48350.037.2518921
CGCAGAG56400.036.89168
TACATGG50950.032.8694532
CTCCGAC6000.032.630848145
ACATGGG51500.031.9553343
GCAGAGT65950.030.8898839
GTGGTAT14700.029.6433331
CATGGGG38800.027.2800944
GAGTACA36450.026.1015851
TGGTATC17100.024.5898912
TCGTATC2701.647386E-724.170998145
ATGCCGC5001.8189894E-1221.753897145
Produced by FastQC (version 0.11.5)