FastQCFastQC Report
Thu 2 Jun 2016
DRR028168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252499
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.047.0689241
TCAACGC30700.046.27784
TGACGCG7250.044.9914145
ATCAACG31700.044.5892753
GTATCAA35300.042.5060421
CAACGCA36350.039.084695
AACGCAG39050.036.5679176
GTACATG68800.035.6109281
TATCAAC42650.033.9911582
TACATGG71750.032.1262662
ACGCAGA43300.031.8068527
CGCAGAG44050.031.2653068
ACATGGG74650.029.7130093
TCTCGGG21400.027.097626145
CTTAGGG27000.025.235918145
GAGTACA51950.025.1155091
GCAGAGT54550.024.1841959
GTATAGG10400.023.0004121
AGTACAT53800.022.9045472
CATGGGG60000.022.7123264
Produced by FastQC (version 0.11.5)