FastQCFastQC Report
Thu 2 Jun 2016
DRR028168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252499
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.10628195617400939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.070.0333941
GTATCAA53550.052.4811361
TCAACGC58600.046.7651444
ATCAACG59350.046.174183
CAACGCA61400.044.514465
AACGCAG64750.042.547296
TATCAAC69150.039.7360572
ACGCAGA74700.036.5888567
CGCAGAG78500.035.0023888
CTCCGAC10450.033.99746145
GTACATG68850.033.9628141
GTGGTAT17400.032.1361051
ACATGGG70300.030.7318743
TACATGG71200.030.6495592
GCAGAGT91250.028.9993389
CATGGGG54750.025.6889134
TGGTATC21300.024.5070292
TAGACCG1801.521346E-424.1661155
GAGTACA51400.024.0180381
CAGAGTA89600.021.2802079
Produced by FastQC (version 0.11.5)