FastQCFastQC Report
Thu 2 Jun 2016
DRR028167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699617
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22850.044.415084
ATCAACG23100.043.6205833
GGTATCA16350.043.450721
GTATCAA25550.041.4238621
CAACGCA26600.039.5161865
GTACATG53550.037.6334461
AACGCAG27750.037.0948836
TACATGG56200.033.7950942
CGACGAC9150.033.274906145
TATCAAC31500.032.6788252
ACATGGG58900.031.1382273
ACGCAGA32750.030.5461487
TGACGCG2901.2732926E-1129.996593145
CGCAGAG33450.029.9069168
TCTCGGG17650.028.750275145
CATGGGG43600.026.7687454
GAGTACA39250.026.5957051
TAGCGTG2001.0669804E-525.3721165
ATCTACC16300.022.68147145
GCAGAGT41300.022.4671829
Produced by FastQC (version 0.11.5)