FastQCFastQC Report
Thu 2 Jun 2016
DRR028167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699617
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.064.253751
GTATCAA39150.053.036341
TCAACGC43900.047.396144
ATCAACG44550.046.8673483
CAACGCA45900.045.4888955
AACGCAG49350.042.308826
TATCAAC51050.040.615882
ACGCAGA56700.036.4407587
CGCAGAG59450.034.6331568
GTACATG53050.033.6658061
TACATGG54300.031.3757152
GCAGAGT69150.028.7265829
ACATGGG56150.028.6634733
GTGGTAT16150.026.9723011
TGGTATC16100.025.6669672
ACCGGTA1450.00122700124.9992878
CTCCGAC6850.024.347382145
GAGTACA41550.024.2875141
CATGGGG41000.023.8712674
AGTACAT42650.022.0978432
Produced by FastQC (version 0.11.5)