FastQCFastQC Report
Thu 2 Jun 2016
DRR028166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117141
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.056.813551
ATCAACG31000.044.427063
GTATCAA31500.044.1821021
TCAACGC30900.044.1016734
GTACATG73100.038.5733951
CAACGCA36000.038.2566345
TACATGG74450.035.4398882
TGACGCG3350.034.6203145
AACGCAG39600.034.595716
TATCAAC41650.033.2409932
ACATGGG77650.032.299093
TTTCGCG2101.4440957E-831.0655369
ACGCAGA43350.030.5997357
CGCAGAG43950.030.0170618
GAGTACA51650.029.3313221
CGACGAA6750.028.994503145
GTACGAC1555.5103978E-528.0591953
AGTACAT53900.026.0896742
CATGGGG58500.024.7816264
GCAGAGT54850.022.7304219
Produced by FastQC (version 0.11.5)