FastQCFastQC Report
Thu 2 Jun 2016
DRR028166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117141
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.10948727552864924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.10707836653298008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.069.57451
GTATCAA54350.053.9584241
TCAACGC61150.047.185644
ATCAACG61800.046.6893463
CAACGCA64150.044.9789855
AACGCAG68150.042.3389856
TATCAAC70800.040.7552262
GTACATG71350.036.5239681
ACGCAGA78850.036.501617
CGCAGAG80600.035.5291868
TACATGG73850.034.3598332
ACATGGG75900.031.807223
GCAGAGT89600.031.3940229
GTGGTAT17850.029.2800431
GAGTACA53750.027.4154241
CTCCGAC8900.025.25851145
CATGGGG56650.025.0829944
AGTACAT57150.023.849272
CAGAGTA86150.022.637079
TGGTATC20650.022.46952
Produced by FastQC (version 0.11.5)