FastQCFastQC Report
Thu 2 Jun 2016
DRR028165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056679
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC16150.060.147125145
TCAACGC28700.049.5058754
ATCAACG28900.049.414113
GTATCAA30750.048.0914271
GGTATCA20450.047.1455761
CGACGAA7450.046.70545145
CAACGCA32850.043.031045
AACGCAG34950.040.6528976
GTACATG68500.036.827421
TATCAAC40050.036.5621262
TACATGG70500.034.0346642
ACGCAGA39850.033.653177
ACATGGG71600.032.4993443
CGCAGAG42100.031.6824178
CATGGGG54800.028.0438674
GAGTACA50750.025.8538421
GCAGAGT52250.024.9728899
GTACGCG1650.00260371421.9668943
AGTACAT53600.021.9095172
GTATAGG9000.020.136321
Produced by FastQC (version 0.11.5)