FastQCFastQC Report
Thu 2 Jun 2016
DRR028165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056679
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.10346777499065242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.075.4918061
GTATCAA50600.051.3569221
TCAACGC57150.045.540244
ATCAACG57350.045.2550133
CAACGCA59900.043.2074365
AACGCAG63050.040.9337926
TATCAAC67300.039.1028752
ACGCAGA73200.034.960757
GTACATG63650.033.7566831
CGCAGAG76700.033.365418
GTGGTAT16250.031.2687931
TACATGG65800.030.9597572
ACATGGG67550.029.6210773
CTCCGAC8300.028.834429145
GCAGAGT87350.028.6334259
CATGGGG53300.023.258734
TGGTATC18750.022.8122352
GAGTACA48300.022.693231
CAGAGTA84500.020.333349
AGTACAT52100.019.8982752
Produced by FastQC (version 0.11.5)