FastQCFastQC Report
Thu 2 Jun 2016
DRR028164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972535
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.044.297831
TCAACGC23150.044.1499634
ATCAACG23350.043.7718053
GTACATG53100.040.5437741
GTATCAA26450.039.737871
CAACGCA27150.037.3783765
TACATGG56500.035.6663442
AACGCAG29350.034.3296176
ACATGGG61850.032.581223
TATCAAC32950.031.6788442
ACGCAGA32250.030.7930747
CGCAGAG33150.029.5197358
GAGTACA40050.024.0719551
CATGGGG47150.023.9830914
GGATAGG15500.023.850676145
TTTCGCG2852.786328E-722.8907419
GCAGAGT43500.021.8295259
AGTACAT43450.020.520012
CATGGGA23700.019.268794
AGAGTAC36250.019.196655
Produced by FastQC (version 0.11.5)