FastQCFastQC Report
Thu 2 Jun 2016
DRR028164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972535
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.10352161051641669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.10012496609692603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.070.390031
GTATCAA45400.055.6480671
TCAACGC49100.050.201674
ATCAACG49200.050.0996363
CAACGCA52550.046.9058465
AACGCAG56200.043.988476
TATCAAC59700.041.773892
ACGCAGA62050.039.2571077
CGCAGAG64150.037.8589828
CTCCGAC8150.036.482044145
GTACATG55800.032.6562421
GCAGAGT77750.030.6772429
TACATGG58900.030.155852
ACATGGG62550.027.2371273
GAGTACA43700.025.4177231
GTGGTAT15700.024.5077171
AGTACAT46650.021.9123132
CAGAGTA75150.021.3198479
CATGGGG47300.019.7719424
TCGTATC3101.46461425E-518.714628145
Produced by FastQC (version 0.11.5)