FastQCFastQC Report
Thu 2 Jun 2016
DRR028162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787415
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC14950.053.8216145
ATCAACG21200.045.8188063
TCAACGC21550.045.7474064
GTATCAA23600.043.002221
CAACGCA23750.041.5097475
GGTATCA17250.039.921731
GTACATG53850.037.2879831
AACGCAG26200.037.074766
TACATGG57100.033.0074582
ACGCAGA29600.032.5712747
TATCAAC31800.031.4576872
ACATGGG59900.030.7384323
CGCAGAG31350.030.2906488
CCGACGC5300.030.089962145
GAGTACA40450.026.5227181
CATGGGG42700.025.8042094
TAGACCG1450.001227862124.9963655
TGACGCG2751.9732033E-723.723822145
AGTACAT42650.023.1150422
ACCGCGT2303.147341E-522.0620088
Produced by FastQC (version 0.11.5)