FastQCFastQC Report
Thu 2 Jun 2016
DRR028162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787415
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.11704053059865784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.11306831373799593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.067.461591
GTATCAA41800.054.5327381
ATCAACG47100.046.6382373
TCAACGC47200.046.539434
CAACGCA49450.044.4218645
AACGCAG51650.042.6700976
TATCAAC55800.039.7564542
ACGCAGA60150.036.2786647
CGCAGAG61600.035.307018
GTACATG52800.031.2101521
GTGGTAT14750.031.0064831
GCAGAGT72100.029.662469
TACATGG52700.029.1639042
TATAACG2054.043577E-728.2915382
ACATGGG54250.027.1279343
CTCCGAC6150.027.12031145
GAGTACA39200.026.8525711
TGGTATC15850.026.5288932
CATGGGG42100.023.5916794
AGTACAT43500.022.8324092
Produced by FastQC (version 0.11.5)