FastQCFastQC Report
Thu 2 Jun 2016
DRR028161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216124
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG17350.049.72153
TCAACGC18050.047.3916174
GTATCAA19800.045.7658541
GGTATCA12100.045.5328641
CAACGCA19850.043.4593475
AACGCAG21800.039.9044656
GACGAAA5000.036.246555145
GTACATG35450.036.1954351
CGCAGAG23750.035.101938
TATCAAC24800.035.0773122
ACGCAGA24350.034.832427
TACATGG36150.032.6870772
ACATGGG40550.029.1402663
GTGGTAT8800.028.0087031
GCCGACA2603.7252903E-927.881966145
GCAGAGT30400.026.231069
GTACCGG1400.001000137725.89046
CATGGGG29100.025.160844
GTATACG2051.2915136E-524.7537461
GTCTCGC3503.5070116E-922.783551
Produced by FastQC (version 0.11.5)