FastQCFastQC Report
Thu 2 Jun 2016
DRR028161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216124
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.15360275761353284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.15352052915656628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.066.37641
GTATCAA32500.055.4075051
TCAACGC37900.047.2475174
ATCAACG38600.046.3906973
CTCCGAC4850.046.34795145
CAACGCA39900.045.0609175
AACGCAG41950.042.8588946
TATCAAC43000.040.9710352
ACGCAGA48000.036.8527457
CGCAGAG49150.035.842978
GTGGTAT10950.032.4924351
CCGTTCG900.00362922332.2209789
GCAGAGT56750.030.659619
GTACATG31000.030.4496271
TGGTATC11450.029.7599012
TACATGG32150.027.737242
ACATGGG33800.026.1676313
CAGAGTA54600.024.9624089
GAGTACA24150.024.3538841
TCGTATC1952.6122402E-422.311419145
Produced by FastQC (version 0.11.5)