FastQCFastQC Report
Thu 2 Jun 2016
DRR028160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021935
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.049.218571
ATCAACG30150.044.7193223
TCAACGC30100.044.311954
GTATCAA31800.043.994641
CAACGCA34850.038.688315
GTACATG68600.038.0406531
TACATGG70250.037.147172
TATCAAC38450.036.385682
AACGCAG38050.036.196666
TCTCGGG19500.033.45627145
ACGCAGA41900.032.0056957
TGACGCG4550.031.86311145
CGCAGAG42750.030.691078
ACATGGG83300.030.4573863
CTTAGGG25400.027.682644145
CATGGGG59100.027.4745184
GAGTACA51000.025.442071
GCAGAGT53450.023.05539
AGTACAT57100.021.3276372
CATGGGA26400.020.5933464
Produced by FastQC (version 0.11.5)