FastQCFastQC Report
Thu 2 Jun 2016
DRR028160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021935
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.13635453167386685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.1328430439158529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.072.6154561
GTATCAA57000.054.2704931
ATCAACG64400.047.1680373
TCAACGC64650.046.9856384
CAACGCA67500.044.8943985
AACGCAG69900.043.352966
TATCAAC72250.042.3452762
ACGCAGA81250.037.02927
CGCAGAG84750.035.414438
GTACATG69750.034.7721331
TACATGG72200.033.437782
GTGGTAT16500.031.686741
ACATGGG77400.030.3475553
GCAGAGT97100.029.9395169
CTCCGAC10200.029.863495145
TAGACCG1358.087585E-426.8507165
CATGGGG60100.025.8142174
GAGTACA53300.024.1143251
GTACGAC1902.1948743E-422.8937683
CAGAGTA92650.022.3789779
Produced by FastQC (version 0.11.5)